Journal: Diagnostics

Article Title: Urinary Epidermal Growth Factor Level as a Noninvasive Indicator of Tubular Repair in Patients with Acute Kidney Injury

doi: 10.3390/diagnostics14090947

Figure Lengend Snippet: Correlation of urinary EGF with renal function and tubular damage markers. Correlations between levels of urinary EGF and NAG ( A ), α1-MG ( B ), β2-MG ( C ), urinary protein ( D ), L-FABP ( E ), NGAL ( F ), KIM-1 ( G ), serum creatinine ( H ), eGFR ( I ), hemoglobin ( J ), and serum EGF ( K ). Data from patients with renal AKI ( n = 83) were used.

Article Snippet: Urinary and serum EGF (DEG00), urinary NGAL (DLCN20), and urinary KIM-1 (DKM100) levels were measured using a Quantikine ® ELISA kit (R&D Systems, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques:

Journal: Diagnostics

Article Title: Urinary Epidermal Growth Factor Level as a Noninvasive Indicator of Tubular Repair in Patients with Acute Kidney Injury

doi: 10.3390/diagnostics14090947

Figure Lengend Snippet: Urinary EGF in living-donor kidney transplantation patients. Serum creatinine ( A ), eGFR ( B ), serum EGF ( C ) levels, urinary EGF ( D ), and the urinary EGF/Cr ratio ( E ) in living-donor kidney transplantation patients after unilateral nephrectomy.

Article Snippet: Urinary and serum EGF (DEG00), urinary NGAL (DLCN20), and urinary KIM-1 (DKM100) levels were measured using a Quantikine ® ELISA kit (R&D Systems, San Diego, CA, USA) following the manufacturer’s instructions.

Techniques: Transplantation Assay

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Mammary Microvessels are Sensitive to Menstrual Cycle Sex Hormones.

doi: 10.1002/advs.202302561

Figure Lengend Snippet: Figure 2. A) Confocal maximum projection images highlight microvessel formation at varied endothelial to fibroblast cell ratios. The gray bars graphically represent the number of fibroblasts and endothelial cells in the different conditions. HMVECs are shown with CellTracker green and microvessels are perfused with Texas Red 70 kDa dextran at day 4 to assess vessel perfusion. Scale bar is 200 μm. Morphologic comparison of the microvessels at the different ratios: B) effective diameter (blue region shown demonstrates average ex vivo mammary-specific measurements), C) mean length, D) branches density, and E) permeability to 70 kDa dextran all for N = 2 biological repeats. F) Semiquantitative measure using a cytokine array of supernatant collected from HMVEC and fibroblast (HMF) cells in monolayer culture. G) MMP1 quantification by ELISA of control media (VL 5%) and supernatant from devices (5:1 ratio) from day 1 to 4. Box plots demonstrate median, percentile 25–75 quartile (box edge) and 10–90 (outer whiskers). Significance is shown by *p < 0.05, **p < 0.01, ***p < 0.001, for data following normality with one-way ANOVA with Tukey means comparison test, and if normality is rejected using Kruskal–Wallis ANOVA test.

Article Snippet: ELISA Assay: Quantification of ET-1, VEGF, bFGF, PLGF, and EGF in the media collected from each device (N = 3 with 3 devices per experiment) was performed respectively with ELISA assay Human Endothelin1 Quantikine ELISA kit (R&D Systems, DET100): Human VEGF Quantikine ELISA Kit (R&D Systems, DVE00), Human FGF basic/FGF2/bFGF Quantikine Kit (R&D Systems, DFB50), Human PlGF Quantikine ELISA Kit (R&D Systems, DPG00), and Human EGF Quantikine ELISA Kit (R&D Systems, DEG00) according to manufacturer’s instructions.

Techniques: Comparison, Ex Vivo, Permeability, Enzyme-linked Immunosorbent Assay, Control

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Mammary Microvessels are Sensitive to Menstrual Cycle Sex Hormones.

doi: 10.1002/advs.202302561

Figure Lengend Snippet: Figure 4. A) Confocal maximum projection images of mammary microvessels formed under different hormonal conditions at day 4. HMVEC shown by CellTracker green and microvessels perfused with Texas Red 70 kDa dextran at day 4 to assess vessel permeability. Scale bar is 200 μm. B) Vessel permeability to 70 kDa dextran is shown for the different hormonal conditions (nontreated, NT; Follicular, Fol; Ovulation, Ovu; Luteal, Lut). Data from five separate experiments with ≥3 devices per condition. ELISA assay performed from supernatant of devices collected at day 2 for C) VEGF, D) FGF, E) EGF, and F) PLGF. Data are from 3 biological repeats with 3 devices per condition each. The dashed red lines in the plots indicate the level of each factors present in the control media. Box plots demonstrate median, red dots for the mean, percentile 25–75 quartile (box edge) and 10–90 (outer whiskers). Significance is shown by *p < 0.05, **p < 0.01, ***p < 0.001, using one-way ANOVA with Tukey means comparison test for data following normality, or if normality is rejected using Kruskal–Wallis ANOVA test.

Article Snippet: ELISA Assay: Quantification of ET-1, VEGF, bFGF, PLGF, and EGF in the media collected from each device (N = 3 with 3 devices per experiment) was performed respectively with ELISA assay Human Endothelin1 Quantikine ELISA kit (R&D Systems, DET100): Human VEGF Quantikine ELISA Kit (R&D Systems, DVE00), Human FGF basic/FGF2/bFGF Quantikine Kit (R&D Systems, DFB50), Human PlGF Quantikine ELISA Kit (R&D Systems, DPG00), and Human EGF Quantikine ELISA Kit (R&D Systems, DEG00) according to manufacturer’s instructions.

Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Control, Comparison